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目的 :观察围急性呼吸窘迫综合征 (ARDS)期循环中性粒细胞(PMN)膜生物物理特性的改变 ,并分析这种改变在脏器炎性损伤发生中的意义。方法 :利用荧光染料染色扫描和阳离子染料染色比色法 ,分别测定正常人、系统性炎症反应综合征 (SIRS)、ARDS及多器官功能障碍综合征 (MODS)患者外周血PMN膜表面水化程度和负电荷量。结果 :SIRS阶段外周血PMN膜水化程度和负电荷量已明显下降 ,至ARDS期已降至低值 ,其后维持于低值。结论 :围ARDS患者外周血PMN明显降低的膜水化程度和膜电荷增加了PMN的相容性 ,使其更易于在肺血管床中附壁滞留 ,从而参与肺组织的炎性损伤过程。
Objective: To observe the change of the biophysical properties of neutrophil (PMN) membrane in the circulatory system of acute respiratory distress syndrome (ARDS) and to analyze the significance of this change in the inflammatory injury of organ. Methods: Fluorescent dye staining and cationic dye staining were used to determine the degree of surface PMN hydration in peripheral blood of patients with normal systemic inflammatory response syndrome (SIRS), ARDS and multiple organ dysfunction syndrome (MODS) And negative charge amount. Results: The degree of PMN membrane hydration and the amount of negative charge in the peripheral blood of SIRS decreased significantly and reached a low level till the ARDS stage and remained low afterwards. CONCLUSIONS: The markedly decreased membrane hydration and membrane charge of PMN in peripheral blood of patients with ARDS increase the compatibility of PMN, make it easier to stay in the pulmonary vascular bed and thus participate in the inflammatory process of lung injury.