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Background: Fluorescence imaging is an attractive diagnostic technique for skin tumour demarcation with potential to move to clinical use. Bi spectral fluorescence imaging combines skin autofluorescence with δ-aminolaevu- linic acid-induced fluorescence. To evaluate the technique, fluorescence data must be compared with the histopathological extent of the tumour, which is the purpose of the current study. Objectives: To investigate the agreement between bispectral fluorescence images and the histopathological tumour boundary of ill-defined basal cell carcinomas (BCCs). After fluorescence imaging the tumours were removed using Mohs micrographic surgery (MMS) to obtain histopathological maps of the tumour boundaries. Methods: Twelve patients with aggressive BCC of mean diameter 16 mm (range 5-32) in the face were included in the study. The patients were subjected to bispectral fluorescence imaging within the 2 months prior to MMS. The fluorescence images and histopathological maps were aligned using image warping. Results: Five patients (42%) showed good agreement with the histopathological mapping and the remaining seven patients (58%) showed partial agreement. Bispectral investigation combining auto-fluorescence with protoporphyrin IX (PpIX) fluorescence generally yielded better agreement with the histopathological boundaries of the tumours compared with using only the PpIX fluorescence. Conclusions: In this preliminary study the fluorescence has been compared with the histopathological tumour boundaries. The result implies that the technique can be applied as a useful tool for indicating tumour boundary of aggressive BCCs. Further refinement is needed to be able to indicate the exact tumour border.
Background: Fluorescence imaging is an attractive diagnostic technique for skin tumor demarcation with potential to move to clinical use. Bi spectral fluorescence imaging combines skin autofluorescence with δ-aminolaevu- linic acid-induced fluorescence. To evaluate the technique, fluorescence data must be compared with the histopathological extent of the tumor, which is the purpose of the current study. Objectives: To investigate the agreement between bispectral fluorescence images and the histopathological tumor boundary of ill-defined basal cell carcinomas (BCCs). After fluorescence imaging the tums were removed using Methods: Twelve patients with aggressive BCC of mean diameter 16 mm (range 5-32) in the face were included in the study. The patients were subjected to bispectral fluorescence imaging within the 2 months prior to MMS. The fluorescence images and histopathological maps were Bispectral investigation combining auto-fluorescence with protoporphyrin IX (PpIX) fluorescence generally yielded better agreement (42%) showed good agreement with the histopathological mapping and the remaining seven patients (58%) showed partial agreement. with the histopathological boundaries of the tumours with using only the PpIX fluorescence. Conclusions: In this preliminary study the fluorescence has been compared with the histopathological boundaries. The result implies that the technique can be applied as a helpful tool for indicating the boundary aggressive BCCs. Further refinement is needed to be able to indicate the exact tumor border.