在过去的二十年中,同种主动脉瓣的临床需求不断增加,因此,必须不断改善组织的消毒和保存方法。据报道,冷冻保存的同种主动脉瓣较4℃抗菌素液保存的同种主动脉瓣在植入前保持较长时间的活力。本研究的目的是论证这二种保存方法是否改变组织的代谢状态。 实验过程 从死亡后9小时内的尸体取得心脏,无菌分离出主动脉瓣,用4℃的乳酸钠Ringer液冲洗,置于加有左旋谷胺酰胺和胎牛血清的RPMI-1640培养液中,进一步 Over the past two decades, the clinical needs for the same type of aortic valve have steadily increased, and the need to continuously improve tissue disinfection and preservation methods. It has been reported that cryopreserved aortas of the same type of aorta preserved in 4 ° C antibiotic fluid retained a relatively long time before implantation. The purpose of this study was to demonstrate whether these two preservation methods alter the metabolic status of the tissue. During the experiment, the heart was obtained from the corpse within 9 hours after the death. The aortic valve was aseptically isolated, rinsed with 4% sodium lactate solution and placed in RPMI-1640 medium supplemented with L-glutamine and fetal bovine serum. further