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随着工业“三废”的排出,农药的普遍使用,环境污染渐趋严重,环境监测已成为令人关注的问题。鱼类直接暴露在水环境中,是理想的实验动物.目前使用一种迅捷而敏感的微核测试法以检测水质具有重要的实践意义。一、微核测试法要点 1.采血。连续吸鳃部或尾部血液0.06～0.1毫升 2.将血小心注入沉降管,把玻璃棒插入血中,在一个方向旋转3～5分钟,将纤维蛋白裹于玻棒上,压紧,小心抽出玻棒,制成抗凝血。
With the release of “three wastes” from industry and the widespread use of pesticides, the environmental pollution has become increasingly serious. Environmental monitoring has become a matter of concern. The direct exposure of fish to the water environment is ideal for experimental animals, and it is of important practical significance to use a rapid and sensitive micronucleus test to detect water quality. First, the main points of micronucleus test method 1. Blood collection. Continuous suction of the gill or the tail of the blood 0.06 ~ 0.1 ml 2. The blood carefully into the sinking tube, the glass rod inserted into the blood, in one direction for 3 to 5 minutes, the fibrin wrapped in glass rods, pressed tightly Glass stick, made of anticoagulant.