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目的阐明低氧在血管壁构形重组中的作用及川芎嗪的疗效机理。方法用细咆计数法及非放射性细胞增殖检测法观察2%低氧对人胚肺成纤维细胞增殖的影响;用Northern印迹杂交法检测2%低氧对人胚肺成纤维细胞Ⅰ型前胶原基因表达的作用及川芎嗪对基因表达的影响。结果低氧24.48及72h能明显促进人胚肺成纤维细胞增殖;低氧24h Ⅰ型前胶原基因表达明显升高;川芎嗪(30μg/ml及10μg/ml)可抑制上述基因表达的升高。结论低氧对人胚肺成纤维细胞有直接利激作用,通过促进其增殖及前胶原基因表达而影响肺血管构形重组和损伤组织的修复。
Objective To elucidate the role of hypoxia in vascular remodeling and the therapeutic mechanism of ligustrazine. Methods The effect of 2% hypoxia on the proliferation of human embryo lung fibroblasts was observed by the fine roger counting method and the non-radioactive cell proliferation assay. The effect of 2% hypoxia on the proliferation of human embryo lung fibroblasts type Ⅰ procollagen Effect of ligustrazine on gene expression. Results Hypoxia 24.48 and 72 h could significantly promote the proliferation of human embryo lung fibroblasts. The expression of 24h type Ⅰ procollagen mRNA was significantly increased in hypoxia group. Ligustrazine (30μg / ml and 10μg / ml) could inhibit the above gene expression. CONCLUSION: Hypoxia can directly stimulate human embryo lung fibroblasts and promote pulmonary vascular remodeling and repair of damaged tissue by promoting its proliferation and procollagen gene expression.