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蛋白酶尤其是ＩＣＥ家族的蛋白酶是细胞死亡机制的核心成分．ＩＣＥ蛋白酶家族中，ＣＰＰ３２（又称Ｙａｍａ，ａｐｏｐａｉｎ）在不同形式的凋亡途径中起核心作用．为深入研究ＣＰＰ３２的结构与功能，克隆了ＣＰＰ３２基因，并在大肠杆菌中进行了表达．采用ＲＴ－ＰＣＲ技术从人肺癌细胞株中获得了ＣＰＰ３２蛋白酶基因．ＤＮＡ序列分析表明，该基因由已报道的编码ＣＰＰ３２αｐ２０亚单位和ＣＰＰ３２βｐ１０亚单位的核苷酸组成，提示ＩＣＥ家族蛋白酶寡聚化可能受ＤＮＡ水平调控．将获得的ＣＰＰ３２基因分别重组到ｐＢＶ３２１和ｐＥＸ３１Ｂ载体上，并分别转化到大肠杆菌中，均获得了ＣＰＰ３２基因的较高表达，表达产物主要以包涵体形式存在．
Proteases, especially proteases of the ICE family, are the core components of cell death. In the ICE protease family, CPP32 (also known as Yama, apopain) plays a central role in different forms of apoptotic pathways. To further study the structure and function of CPP32, the CPP32 gene was cloned and expressed in E. coli. The CPP32 protease gene was obtained from human lung cancer cell line by RT-PCR. DNA sequence analysis showed that the gene consists of nucleotides that have been reported to encode CPP32αp20 subunits and CPP32βp10 subunits, suggesting that oligomerization of ICE family proteases may be regulated by DNA levels. The obtained CPP32 gene was recombined into pBV321 and pEX31B vectors, respectively, and transformed into E. coli, respectively, all obtained higher expression of CPP32 gene, the expression products mainly in the form of inclusion bodies.