论文部分内容阅读
目的探讨β-榄香烯(-βelemene)对多药耐药细胞株K562/阿霉素(ADM)的耐药逆转及其对该细胞P-糖蛋白(P-gp)表达的影响。方法采用MTT法检测细胞的药敏性及耐药逆转,免疫组织化学及免疫电镜观察P-gp的表达,流式细胞术进行定量分析,应用SPSS(10.0 production pacility)软件包对实验结果进行统计学处理。结果1.非细胞毒性浓度的-βelemene(4.0 mg/L)可显著降低ADM对K562/ADM细胞的IC50(P<0.01),逆转倍数为2.18倍;2.P-gp在耐药细胞株K562/ADM中呈高表达,而在敏感细胞株K562中表达很低,进一步定位研究结果表明,P-gp主要分布于细胞膜上;3.非细胞毒性浓度的-βelemene(4.0 mg/L)能够显著降低耐药细胞P-gp的表达。结论-βelemene能够明显逆转K562/ADM细胞对ADM的耐药性,降低该细胞P-gp的表达是其主要逆转机制之一。
Objective To investigate the reversal of multidrug resistance in β-elemene (ADM) cell line K562 / ADM and its effect on the expression of P-glycoprotein (P-gp). Methods MTT assay was used to detect the drug sensitivity and reversal of drug resistance. The expression of P-gp was observed by immunohistochemistry and immunoelectron microscopy. The flow cytometry was used for quantitative analysis. The SPSS (10.0 production pacility) software package was used to analyze the experimental results Learn to deal with. The results showed that IC50 of ADM on K562 / ADM cells was significantly decreased by non-cytotoxic concentration of -βelemene (4.0 mg / L), with a 2.18-fold reversal fold. 2. The expression of P-gp in the resistant cell line K562 / ADM, while the expression in sensitive cell line K562 was very low. Further localization results showed that P-gp mainly distributed in the cell membrane.3. The non-cytotoxic concentration of -βelemene (4.0 mg / L) Reduce the expression of P-gp in drug-resistant cells. Conclusion -βelemene can reverse the drug resistance of K562 / ADM cells to ADM, and reduce the expression of P-gp in the cells is one of the main reversal mechanisms.