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取河套蜜瓜 (Cucumis melo L .cv Hetao)在 MS培养基上生长至 5 d的幼嫩子叶 ,在 MS+ 6 - BA0 .5 +IAA1 .0 的培养基中直接诱导生芽 ,待再生芽长至 0 .4cm～ 0 .6 cm时 ,切下转入芽伸长培养基中 (MS+ 6 - BA0 .9) ,待芽伸长至 0 .8cm～ 1.2 cm时 ,再转入生根诱导 (MS+ IAA2 )中 ,待植株长至 8cm～ 9cm时 ,转入沙质土壤中炼苗。整个过程约需 6 5 d～ 75 d,单个外植体诱导成芽最多达 31个 ,再生植株诱导率可达 6 2 .5 % ,为河套蜜瓜的快速繁殖和遗传转化提供了良好的再生系统。
The young cotyledons of Cucumis melo L. Cv Hetao were grown on MS medium for 5 days. The buds were directly induced in MS + 6 - BA0. 5 + IAA1. (MS + 6 - BA0. 9) was cut and the elongation of the shoots was increased from 0.8 cm to 1.2 cm, and then transferred to rooting induction (MS + IAA2), until the plants grow to 8cm ~ 9cm, transferred to sandy soil refining. The whole process took about 65-55 days. Up to 31 shoots could be induced from a single explant, and the induced rate of regenerated plants reached 62.5%, which provided a good regeneration for rapid propagation and genetic transformation of Hetao melon system.