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目的分析酶联免疫吸附试验法和荧光定量PCR检测患者感染丙型肝炎病毒的情况,以期为丙型肝炎病毒的检测寻找更为合理有效的检测方法。方法选择2013年1月-2015年12月在该中心体检的体检者750例作为检测对象,分别采用酶联免疫吸附试验(ELISA)和荧光定量PCR进行检测,统计分析检测结果。结果 ELISA法检出阳性21例,阳性率为2.80%,PCR法检出阳性40例,阳性率为5.33%,两种检测方法检测结果比较差异有统计学意义(χ~2=6.170,P<0.05)。结论荧光定量PCR检测方法用于检测丙型肝炎病毒感染准确可靠,可在临床检测中推广使用。
Objective To analyze the status of hepatitis C virus (HCV) infection by enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative PCR (PCR) in order to find a more reasonable and effective method for the detection of hepatitis C virus. Methods A total of 750 physical examinations from January 2013 to December 2015 in our center were selected as the testing objects. The samples were detected by enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR, and the results were statistically analyzed. Results The positive rate was 2.80% by ELISA and the positive rate was 2.80%. The positive rate was 5.33% by PCR. The difference between the two methods was statistically significant (χ ~ 2 = 6.170, P < 0.05). Conclusion The method of fluorescence quantitative PCR for detection of hepatitis C virus infection is accurate and reliable, and can be widely used in clinical testing.