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目前,在无机离子交换色谱中,多使用电化学检测系统,今年我国也研制成了库仑检测器。我们应用了高效离子交换柱,实现了某些离子的分离;并选用了适当的螯合剂与流出组分进行了柱后螯合显色反应;用一般高效液相色谱仪常备的分光光度检测器,实现了某些无机离子的检测。这种方法既增强了检测某些离子的选择性,也提高了检测灵敏度。实践表明,用Partisil-10 SCX键合相离子交换柱进行分离,用水溶性二乙醇胺二硫代甲酸螯合剂为柱后反应试剂,检测波长为405毫微米,测得Cu(Ⅱ)、Zn(Ⅱ)、Ni(Ⅱ)、Co(Ⅱ)、Fe(Ⅱ)的检出限量为毫微克级。实验液相色谱仪Perkin-Elmer Series 3型,配有LC-65T紫外-可见分光光度检
At present, inorganic ion exchange chromatography, the use of electrochemical detection system, this year our country has also developed a Coulometric detector. We use a high efficiency ion exchange column, to achieve the separation of certain ions; and the selection of a suitable chelating agent and effluent components were post-column chelate color reaction; with conventional high performance liquid chromatography standing spectrophotometric detector , To achieve the detection of some inorganic ions. This method not only enhances the selectivity of detecting certain ions, but also improves the detection sensitivity. Practice shows that the Partisil-10 SCX bonded phase ion exchange column was used for separation. The water-soluble diethanolamine dithiocarbamate chelating agent was used as the post-column reagent, and the detection wavelength was 405 nm. The contents of Cu (Ⅱ), Zn ), Ni (Ⅱ), Co (Ⅱ), Fe (Ⅱ) detection limit of nanograms. Experimental Liquid Chromatograph Perkin-Elmer Series 3 with LC-65T UV-Vis spectrophotometry