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本研究应用体外细胞毒试验，小鼠结肠癌移植瘤及裸鼠移植人大肠癌模型，观察博来霉素A6（A6）和平阳霉素（PYM）对大肠癌的疗效。结果发现2种药物在体外对人大肠癌细胞有较强的活性。在1／9LD50等毒性剂量下，A6和PYM对小鼠结肠癌26皮下和盲肠移植瘤的生长有明显的抑制作用，2次实验结果：对小鼠结肠癌皮下移植瘤，A6的抑瘤率分别为87％和88％，PYM为86％和91％，丝裂霉素C（MMC）为64％和25％，5—氟脲嘧啶（5—FU）为45％和27％；对盲肠移植瘤，A6抑瘤率分别为99％和97％，PYM为100％和97％，MMC为77％和25％，5—FU均为44％。同样A6和PYM对裸鼠移植的人结肠癌HT－29的生长也有较强的抑制作用，A6对肿瘤生长的抑制率为82％，PYM为78％，MMC为53％，5—FU为12％。表明A6和PYM对小鼠结肠癌的裸鼠移植的人结肠癌的生长抑制作用均明显较MMC和5—FU为强，A6的作用似略较PYM为强，但二者间无明显差别；在抑制肿瘤生长的同时，A6和PYM还使肿瘤的组织学发生变化，肿瘤坏死明显增加和核分裂数减少。在治疗剂量下A6和PYM对带瘤小鼠和荷瘤裸鼠的骨髓有核细胞数均无明显影响。
In this study, we used in vitro cytotoxicity test, mice colon cancer xenografts and nude mice transplanted human colorectal cancer models to observe the efficacy of bleomycin A6 (A6) and Pingyangmycin (PYM) on colorectal cancer. As a result, it was found that the two drugs have strong activity against human colorectal cancer cells in vitro. In the 1/9LD50 and other toxic doses, A6 and PYM significantly inhibited the growth of mice with colon cancer26 subcutaneous and caecal tumors. Two experimental results: the tumor inhibition rate of mouse subcutaneous tumors of colon cancer, A6 They were 87% and 88%, PYM was 86% and 91%, mitomycin C (MMC) was 64% and 25%, 5-fluorouracil (5-FU) was 45% and 27%; In the transplanted tumors, the tumor inhibition rates of A6 were 99% and 97%, PYM was 100% and 97%, MMC was 77% and 25%, and 5-FU was 44%. Similarly, A6 and PYM also had a strong inhibitory effect on the growth of human colon cancer HT-29 transplanted in nude mice. The inhibitory rate of A6 on tumor growth was 82%, PYM was 78%, MMC was 53%, and 5-FU was 12 %. It was shown that the growth inhibition effects of A6 and PYM on colon cancer in nude mice transplanted with human colon cancer were significantly stronger than those of MMC and 5-FU, and the effect of A6 was slightly stronger than that of PYM, but there was no significant difference between them. While inhibiting tumor growth, A6 and PYM also caused changes in the histology of tumors, marked increases in tumor necrosis and reductions in the number of mitoses. At therapeutic doses, A6 and PYM had no significant effect on the number of bone marrow nucleated cells in tumor-bearing mice and tumor-bearing nude mice.