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为观察苯肾上腺素(phenylephrine,Phen)、内皮素-1(endothelin-1,ET-1)和KCl引起大鼠肺动脉主干与三级分支间收缩反应的差异,本文分别用传统的大血管张力检测技术和DMT微血管张力检测技术,记录KCl、Phen和ET-1引起的去内皮肺动脉主干和三级分支血管张力变化。结果显示:Phen可引起肺动脉主干显著收缩,但是不引起肺动脉三级分支的明显收缩;与肺动脉主干相比,ET-1导致肺动脉三级分支的收缩反应减弱,反应的最大值和亲和力指数(pD_2)值均降低;与肺动脉主干相比,KCl引起肺动脉三级分支的收缩反应增强。上述结果说明,肺动脉主干和肺动脉三级分支的血管反应性存在差异,进行肺小动脉血管功能研究具有重要意义。本实验可为进一步研究肺动脉小分支的血管功能及其在肺高压中的改变提供重要实验依据。
To observe the differences of contractile response between the main pulmonary artery and the tertiary branches in rats induced by phenylephrine (Phen), endothelin-1 (ET-1) and KCl, we used traditional blood vessel tension Technology and DMT microvascular tension testing technology to record KCl, Phen and ET-1-induced endothelium of the pulmonary artery trunk and tertiary branches of vascular tension changes. The results showed that: Phen can cause significant contraction of the pulmonary artery trunk, but does not cause obvious contraction of the pulmonary artery tertiary branches; compared with the pulmonary artery, ET-1 lead to decreased pulmonary artery tertiary contraction response, the maximum response and affinity index (pD_2 ) Values were decreased; compared with the pulmonary artery, KCl caused pulmonary artery tertiary contraction response. The above results indicate that there are differences in the vascular reactivity between the pulmonary artery and the tertiary branches of pulmonary arteries, and it is of great significance to study the function of pulmonary arterioles. This experiment can provide important experimental evidences for further study on the vascular function of the pulmonary artery branch and its changes in pulmonary hypertension.