论文部分内容阅读
目的 :探讨人乳头瘤病毒 (HPV) 1 6在舌癌细胞中存在的形式 ,以分析该病毒在舌癌发生中的意义。方法 :采用 Southern blot杂交技术对 2 0例新鲜舌癌组织进行 HPV1 6型基因组 DNA存在状态分析。结果 :2 0例舌癌中有 5例检测出 HPV1 6病毒基因序列 ,该病毒在舌癌中主要以游离形式存在于细胞基因组之外。结论 :HPV1 6在舌癌发生中的作用不是通过病毒基因与细胞基因组整合产生的。推测 HPV某些基因产物与靶细胞某些基因相互作用可能是该病毒致病的途径
Objective: To investigate the existence of human papillomavirus (HPV) 1 6 in tongue cancer cells in order to analyze the significance of this virus in tongue carcinogenesis. Methods: Southern blot was used to detect the presence of HPV16 genomic DNA in 20 fresh tongue cancer tissues. Results: Twenty five cases of tongue cancer detected HPV16 gene sequence. The virus mainly exists in the form of free form outside the cell genome in tongue cancer. Conclusion: The role of HPV16 in tongue carcinogenesis is not through the integration of the viral genes with the cellular genome. It is speculated that certain gene products of HPV interact with certain genes of target cells may be pathogenic pathways of the virus