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Inheritance of line Jinghe891-1 resistant to pathotype of Puccinia striiformis in two patterns of temperature (Normal: day 18℃/night 10℃, High: day 24℃/night 15℃ )was studied in this paper. The results showed that there were at least two pairs of dominant major genes and one pair of recessive minor genes in Jinghe 891-1. The two pairs of major genes that conferred resistance to CY31 were allelic or linked closely with resistance gene in Jubilejna Ⅱ , Kangyin655 and T. spelta Album. They were novel resistance genes and were inherited in a repeated or independent mode. The minor genes, which could modify the major genes,were sensitive to temperature and conferred resistance to all pathotypes of Puccinia striiformis in China. It is recommended that this line can be used as an important resource stock.