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目的:观察脂多糖对人牙周膜成纤维细胞COX-2表达的影响,探讨牙周病发生发展过程中脂多糖(LPS)与COX-2的关系。方法:使用不同浓度LPS刺激体外培养人牙周膜成纤维细胞,免疫组织化学法检测细胞COX-2蛋白表达,灰度分析并进行统计学处理。结果:LPS组COX-2蛋白表达明显高于对照组,并且LPS浓度越高,COX-2表达越强。结论:LPS能够诱导人牙周膜成纤维细胞COX-2表达上调,随着LPS刺激浓度增高,COX-2表达逐渐增强,这一机制很可能在牙周炎发生发展过程中扮演重要角色。
Objective: To observe the effect of lipopolysaccharide on the expression of COX-2 in human periodontal ligament fibroblasts and to explore the relationship between lipopolysaccharide (LPS) and COX-2 during the development of periodontal disease. Methods: Human periodontal ligament fibroblasts were cultured in vitro with different concentrations of LPS. The expression of COX-2 protein in the cells was detected by immunohistochemistry. The gray scale analysis and statistical analysis were performed. Results: The expression of COX-2 protein in LPS group was significantly higher than that in control group. The higher the concentration of LPS, the stronger the expression of COX-2. CONCLUSION: LPS can up-regulate the expression of COX-2 in human periodontal ligament fibroblasts. With the increase of LPS concentration, the expression of COX-2 gradually increases. This mechanism is likely to play an important role in the development of periodontitis.