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采用反相高效液相色谱内标法测定卷烟烟气总粒相物中的烟碱含量，内标物为对氨基苯磺酸，方法的平均回收率为９９．７％～１００．１％，精密度好，操作简便、快速，可作为检测烟碱的一种可靠方法。
The content of nicotine in cigarette smoke total particulate matter was determined by RP-HPLC. The internal standard was p-aminobenzenesulfonic acid. The average recoveries of the method were 99.7% -100.1% Precision, easy to operate, fast, as a reliable method of testing nicotine.