Objective:To study the antimicrobial,hemolytic activity and immunomodulatory activity of fish epidermal mucus and their chemical constituents from Cynoglossus arel(C.arel) and Alius caelatus(A.caelatus).Mucus plays an important role in the prevention of colonization by parasites,bacteria and fungi.Methods:Epidermal mucus was obtained from two marine fishes,lyophilized and the chemical composition of epidermal mucus was analysed by FT-IR analysis.The in vitro antimicrobial activity against human pathogens(fungi,gram positive and gram-negative bacteria) and also the hemolytic activity and immunomodulatory activity were determined.Results:Totally ten human pathogens were tested against the fish mucus.Out of the ten pathogens,five pathogens have proved to be sensitive to the mucus.Maximum zone of inhibition was observed against Vibrio cholera(V.cholera)(9 mm and 2 mm in diameter),followed by Staphylococcus aureus(S.aureus) with a inhibition zone of(6 mm and 3 mm),Streptococcus areus(S.areus)(5 mm and 4 mm),Vibrio parahemolyticus(V.parahemolyticus)(4 mm and 5 mm) respectively.Conclusions:The present investigation has revealed that positive progresses in the fish mucus extracts against human pathogens and hemolytic activity.But further efforts are required for the purification and isolation of the active antimicrobial compounds in order to establish their possible applications. Objective: To study the antimicrobial, hemolytic activity and immunomodulatory activity of fish epidermal mucus and their chemical constituents from Cynoglossus arel (C.arel) and Alius caelatus (A. caelatus). Mucus plays an important role in the prevention of colonization by parasites, bacteria and fungi. Methods: Epidermal mucus was obtained from two marine fishes, lyophilized and the chemical composition of epidermal mucus was analyzed by FT-IR analysis. The in vitro antimicrobial activity against human pathogens (fungi, gram positive and gram-negative bacteria) and also the hemolytic activity and immunomodulatory activity were determined. Results: Totally ten human pathogens were tested against the fish mucus. Of the ten pathogens, five pathogens have proved to be sensitive to the mucus. Maximum zone of inhibition was observed against Vibrio cholera (9 mm and 2 mm in diameter), followed by Staphylococcus aureus (S. aureus) with a inhibition zone of (6 mm and 3 mm), Streptococcus areus (5 mm and 4 mm), Vibrio parahemolyticus (V. parahemolyticus) (4 mm and 5 mm) respectively. Conclusions: The present investigation has revealed that positive progresses in the fish mucus extracts against human pathogens and hemolytic activity. Bat further efforts are required for the purification and isolation of the active antimicrobial compounds in order to establish their possible applications.