论文部分内容阅读
目的 :探讨腺病毒介导的小鼠γ 干扰素在小鼠哮喘模型肺上皮细胞内的转基因表达对过敏原所致的嗜酸性粒细胞浸润的作用。方法 :C5 7小鼠经卵蛋白 (ovalbumin ,OVA)腹腔致敏和气道吸入激发建立哮喘模型 ,48h后收获小鼠肺泡灌洗液 (bronchoalveolarlavage ,BAL)和小鼠肺 ;哮喘模型在OVA激发前 48h ,在其气道内给予带有γ 干扰素的复制缺陷腺病毒 (replication deficientadenoviruswithIFN γgene,AdCMVmIFNγ) 5× 10 8空斑形成单位 (pfu) ,同上在OVA激发 48h后收获其肺泡灌洗液和肺。结果 :在卵蛋白所致的哮喘模型中 ,肺组织病理可见支气管周围、血管周围及部分肺泡内明显的嗜酸粒细胞浸润 ,肺泡灌洗液中的嗜酸粒细胞平均占 (75 .13±6 .85 ) % ,而在阴性对照组中则未见嗜酸粒细胞 ;小鼠哮喘模型气道内给予AdCMVmIFNγ后 ,肺内嗜酸粒细胞浸润的程度明显减少 ,肺泡灌洗液中的嗜酸粒细胞占 (9.0 0± 4.5 8) % (P <0 .0 0 1) ,二者均显著低于哮喘模型组。结论 :小鼠的IFN γ经腺病毒介导在小鼠肺上皮细胞内的表达可明显抑制过敏原所致的肺内嗜酸性粒细胞浸润 ,从而为进一步利用细胞因子的体内转基因免疫治疗过敏性哮喘探索了新的治疗途径
OBJECTIVE: To investigate the effect of adenovirus-mediated transgene expression of mouse interferon gamma on the allergen-induced eosinophil infiltration in mouse lung asthma model lung epithelial cells. Methods: The asthmatic model was established by intraperitoneal sensitization and airway inhalation of ovalbumin (OVA) in C5 7 mice. BALB / c mice were harvested at 48 h. The asthmatic model was induced by OVA challenge 48h, 5 × 10 8 plaque forming units (pfu) of replication deficient adenovirus with IFNγgene (AdCMVmIFNγ) were administered to the airway, and their bronchoalveolar lavage fluid and lungs were harvested 48 hours after OVA challenge . Results: In the model of asthma induced by ovalbumin, obvious eosinophil infiltration around the bronchi, perivascular and part of the alveoli was found in the lung tissue pathology. The average number of eosinophils in BALF was (75.13 ± 6 .85)%, while in the negative control group, no eosinophils were found. Advice of administration of AdCMVmIFNγ in the asthmatic mouse model significantly decreased the degree of infiltration of eosinophils in the lung. The level of eosinophils in the alveolar lavage fluid Granulocytes accounted for (9.0 ± 4.58)% (P <0.001), both of which were significantly lower than those in asthma model group. CONCLUSION: The expression of IFN-γ in mouse lung epithelial cells induced by adenovirus can significantly inhibit the eosinophil-induced eosinophil infiltration in mice and thus make it more useful for the further immunization of transgenic mice with cytokines Asthma explores new avenues of treatment