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以从土壤中分离、筛选出的X4菌株作为分子识别元件,采用夹层法制备微生物膜并与极谱型氧电极组成了呼吸抑制型毒性微生物传感器。实验确定了传感器的最佳工作条件:温度为30℃,pH范围为7.0~7.5;以HgCl2作为参比毒物时,微生物传感器标准曲线为y=1.05 x+0.21,线性相关系数为0.9933,线性范围为0.1~1.97 mg/L,相对标准偏差为1.8%。用该微生物传感器对某印染废水的毒性进行测试,该废水的毒性相对于HgCl2的浓度为1.69 mg/L,属于剧毒,与发光细菌法进行比对,测定结果具有一致性。
The X4 strain isolated from soil and screened out was used as a molecular recognition element. The sandwich membrane method was used to prepare the microbial membrane and formed a respiratory-inhibitory toxic microbial sensor with polarographic oxygen electrode. The optimum working conditions of the sensor were determined experimentally. The temperature range was 30 ℃ and the pH range was 7.0-7.5. The standard curve of the biosensor was y = 1.05 x + 0.21 and the linear correlation coefficient was 0.9933 with HgCl2 as reference poison. The linear range 0.1 ~ 1.97 mg / L, the relative standard deviation was 1.8%. The toxicity of a printing and dyeing wastewater was tested by using the microbial sensor. The toxicity of the wastewater was 1.69 mg / L relative to HgCl2, which was highly virulent and was compared with the luminescent bacteria method. The results were consistent.