论文部分内容阅读
目的观察五肽胃泌素（ＰＧ）、胃泌素受体拮抗剂丙谷胺（ＰＧｌ）和生长抑素（ＳＳ）长效类似物ＳＭＳ２０１＿９９５对体外培养人大肠癌细胞系ＳＷ１１１６生长的影响。方法设空白组、对照组、ＰＧ组、ＰＧｌ组、ＳＳ组、ＰＧｌ＋ＰＧ组和ＳＳ＋ＰＧ组。各组主要药物浓度分５×１０－５＿５×１０－１０ｍｏｌ／Ｌ６个浓度，联合用药组ＰＧ浓度均为５×１０－５ｍｏｌ／Ｌ。采用ＭＴＴ比色分析法检测细胞增殖情况。结果ＰＧ能提高细胞增殖率；ＰＧｌ则抑制细胞增殖；ＰＧ对ＰＧｌ的抑制率总体影响不大，而使其与ＰＧｌ浓度对数值呈显著负相关（ｒ＝－０．８３，Ｐ＜０．０５）。ＳＳ在低浓度时促进细胞增殖，而高浓度时则有抑制作用，细胞增殖率与药物浓度对数值呈显著负相关（ｒ＝－０．９１，Ｐ＜０．０５）；ＰＧ可逆转ＳＳ的促增殖作用，并使其抑制率有所提高，且细胞增殖率仍与ＳＳ浓度对数值呈显著负相关（ｒ＝－０．８４，Ｐ＜０．０５）。结论激素可控制大肠癌的生长，但尚有许多问题待阐明
Objective To observe the effects of pentagastrin (PG), gastrin receptor antagonist proglumide (PGl) and somatostatin (SS) long-acting analogue SMS201_995 on the growth of human colorectal cancer cell line SW1116 in vitro. Methods Blank group, control group, PG group, PGl group, SS group, PGl+PG group and SS+PG group were set up. The concentration of the main drug in each group was 5×10-5_5×10-10 mol/L, and the concentration of PG in the combination group was 5×10-5 mol/L. MTT assay was used to detect cell proliferation. Results PG could increase the cell proliferation rate; PG1 inhibited cell proliferation; PG had little effect on the inhibition rate of PG1, and it had a significant negative correlation with the logarithm of PG1 concentration (r=-0.83, P<0.05). ). SS promoted cell proliferation at low concentrations, but inhibited proliferation at high concentrations, and the cell proliferation rate was significantly negatively correlated with drug concentration logarithm (r=-0.91, P<0.05); PG reversed SS Proliferation was promoted and the inhibition rate was increased, and the cell proliferation rate was still significantly negatively correlated with the SS concentration logarithm (r=-0.84, P<0.05). Conclusion Hormones can control the growth of colorectal cancer, but there are still many problems to be clarified