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研究结果表明,用0.5%的秋水仙素处理橡胶树二倍体花药体细胞愈伤组织(浸泡或滴渍)1d可有效地从多倍性的细胞再分化成多倍性胚状体并再生植株。所诱导培育获得的多倍胚状体及植株,通过细胞学鉴定表明具有多倍体表型特征的,其体细胞染色体数为2n=72,未见嵌合现象,对照的2n=36。
The results showed that treatment of 0.5% colchicine on diploid anther somatic calluses (soaked or dripped) for 1 day effectively re-differentiated from polyploid cells into polyploid embryoid bodies Regenerate plants. The polyembryonic embryos and plants induced by induction showed cytological characteristics of polyploid phenotype. The number of somatic chromosomes was 2n = 72, and no chimerism was observed. The control 2n = 36.