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To characterize and purify the allergen extracted from Amaranthus viridis pollen.Extracts of Amaranthus viridis pollen was prepared and analyzed by SDS-PAGE.The protein bands were visualized by staining the gel with Coomassis blue and analyzed by immuno-blotting with serum from 30 allergic patients and from 20 normal people.30 protein bands were detected in SDS-PAGE.2 of these bands with molecular weight of 75 kD a and 67 kD a,showed immumo-reactivity with IgE in the sera from patients with allergy to Amaranthus viridis pollen.The 75 kD a and 67 kD a protein was the major allergen.Meamwhile,Ion-exchange chromatography with DE-52 was used for purification of the antigens.The purified products were characterized by Westerm-blotting.Result from the Ion-exchange chromatography indicated that the pollen allergens(75 kD a and 67 kD a)from the Amaranthus viridis pollen were eluted in theⅠandⅡpeak.The study shows pollen extracts from Amaranthus viridis pollen was purified and characterized and the major allergen was 75 kD a and 67 kD a protein.
To characterize and purify the allergen extracted from Amaranthus viridis pollen. Extracts of Amaranthus viridis pollen was prepared and analyzed by SDS-PAGE. The protein bands were visualized by staining the gel with Coomassis blue and analyzed by immuno-blotting with serum from 30 allergic patients and from 20 normal people. 30 protein bands were detected in SDS-PAGE. 2 of these bands with molecular weight of 75 kD a and 67 kD a, showed immumo-reactivity with IgE in the sera from patients with allergy to Amaranthus viridis pollen. The 75 kD a and 67 kD protein was the major allergen. Meamwhile, Ion-exchange chromatography with DE-52 was used for purification of the antigens. The purified products were characterized by Westerm-blotting. Result from the Ion-exchange chromatography that the pollen allergens (75 kD a and 67 kD a) from the Amaranthus viridis pollen were eluted in the I and II peak. The study shows pollen extracts from Amaranthus viridis pollen was purified and characterized and the major allergen was 75 kDa and 67 kD a protein.