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目的:探讨硫酸软骨素对慢性酒精中毒脑损伤的作用及可能机制。方法:雄性Wistar大鼠60只随机分为6组,酒精模型组以剂量为8 ml.kg-.1d-1 50%的酒精每天灌胃一次,纳洛酮药物组给予乙醇半小时后腹腔注射纳洛酮0.08 mg.kg-1.d-1,硫酸软骨素低、中、高剂量干预组在酒精模型组的基础上分别给予硫酸软骨素50、100、150 mg.kg-1.d-1,空白对照组给予等体积的蒸馏水,持续2周;第三周把50%的酒精的剂量递增为12 mg.kg-.1d-1,持续灌胃6周。在第八周末实验结束后取血,分离血清,留取脑组织。HE染色观察各组大鼠神经细胞的变化。生化测定各组大鼠血清及脑组织匀浆中谷胱甘肽过氧化物酶(GSH-PX)和超氧化物歧化酶(SOD)的活性以及脂质过氧物终末产物丙二醛(MDA);并测定脑皮质中β-内啡肽含量(β-EP)。结果:模型组大鼠大脑皮质和海马区神经元数量明显减少,神经细胞排列紊乱。硫酸软骨素中剂量组大鼠大脑皮质和海马区神经细胞排列层次较清晰。与酒精组相比较,硫酸软骨素中剂量组大鼠血清和脑组织匀浆中MDA含量明显降低(P<0.01),脑皮质中β-内啡肽含量明显降低(P<0.01);GSH-PX含量及SOD活性显著升高(P<0.01)。结论:硫酸软骨素对大鼠慢性酒精中毒脑损伤具有保护作用。
Objective: To investigate the effect of chondroitin sulfate on brain injury induced by chronic alcoholism and its possible mechanism. Methods: Sixty male Wistar rats were randomly divided into 6 groups. The model rats in the alcohol group were orally administered with a dose of 8 ml.kg-1d-1 50% alcohol once a day. The naloxone-treated rats were given ethanol for half an hour and then injected intraperitoneally Naloxone 0.08 mg.kg-1.d-1, low, middle and high dose of chondroitin sulfate intervention group were given chondroitin sulfate 50, 100, 150 mg.kg-1.d- 1, the control group was given an equal volume of distilled water for 2 weeks; the third week the dose of 50% alcohol was increased to 12 mg.kg-.1d-1, continued for 6 weeks. At the end of the eighth weekend after the experiment, blood was collected, serum was separated and brain tissue was collected. The changes of nerve cells in each group were observed by HE staining. Biochemical determination of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activity in serum and brain homogenates and lipid peroxidation of malondialdehyde (MDA) ); And β-endorphin content (β-EP) was measured in the cortex. Results: The number of neurons in the cerebral cortex and hippocampus in model group decreased significantly, and the nerve cells arranged in disorder. Chondroitin sulfate medium dose group rat cerebral cortex and hippocampus nerve cells arranged more clearly. Compared with alcohol group, the content of MDA in serum and brain homogenate of rats in medium dose of chondroitin sulfate decreased significantly (P <0.01), and the content of β-endorphin in cerebral cortex decreased significantly (P <0.01) PX content and SOD activity were significantly increased (P <0.01). Conclusion: Chondroitin sulfate has a protective effect on brain injury induced by chronic alcoholism in rats.