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景天三七广泛用于治疗各种出血、失眠、创伤肿痛,但关于该药抗炎活性尚未见报道。本研究的目的是基于炎症细胞模型探讨景天三七提取物包括石油醚、醋酸乙酯及水部位的抗炎活性。结果显示,富含酚酸和黄酮类成分的景天三七醋酸乙酯部位能显著抑制脂多糖诱导的RAW 264.7细胞中NO,TNF--α,IL--6的释放(P<0.01),表明其醋酸乙酯部位具有潜在的抗炎活性,同时建立该抗炎活性部位的HPLC指纹图谱。HPLC指纹图谱分析显示其抗炎活性可能与其所含的没食子酸、原儿茶酸、对羟基苯甲酸、没食子酸乙酯、鸢尾酚酮、5,7--二羟基色原酮、槲皮苷、槲皮素、木犀草素、山奈酚和异鼠李素等成分有关。综上,景天三七醋酸乙酯部位体外具有抗炎活性,是一潜在的天然抗炎剂。
Sedum thirty-seven widely used in the treatment of various bleeding, insomnia, traumatic swelling and pain, but the anti-inflammatory activity of the drug has not been reported. The purpose of this study was to investigate the anti-inflammatory activity of extracts of P. euphratica, including petroleum ether, ethyl acetate and water, based on the inflammatory cell model. The results showed that ethyl acetate fraction, which is rich in phenolic acids and flavonoids, could significantly inhibit the release of NO, TNF - α and IL - 6 from RAW 264.7 cells induced by lipopolysaccharide (P <0.01) Indicating that the ethyl acetate fraction has potential anti-inflammatory activity, while establishing the HPLC fingerprint of the anti-inflammatory active site. HPLC fingerprinting analysis showed that its anti-inflammatory activity may be related to its contained gallic acid, protocatechuic acid, p-hydroxybenzoic acid, ethyl gallate, iris ketone, 5,7-dihydroxy chromogen, quercetin , Quercetin, luteolin, kaempferol and isorhamnetin and other ingredients related. In summary, Sedum palmatum ethyl acetate in vitro anti-inflammatory activity, is a potential natural anti-inflammatory agent.