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目的:对中药有效成分绞股蓝甙(GP),黄芩甙(BC),丹参酚酸(SM),灯盏花素(EB)以及复方(黄芩甙+灯盏花素)干预4NQO诱导的大鼠舌癌模型进行动态研究,评价其疗效。方法:在4NQO诱导的大鼠舌癌模型基础上,将150只大鼠随机分成8组(绞股蓝甙组,黄芩甙组,丹参酚酸组,灯盏花素组,黄芩甙+灯盏花素1组,黄芩甙+灯盏花素2组,正常对照组,癌变模型组),于12~16周分别处死动物,采用组织病理学方法和免疫组织化学方法(增殖细胞核抗原)检测组织标本,利用SAS统计软件进行数据处理,方差分析进行统计。结果:组织病理学结果显示:中药干预组的上皮异常增生程度明显低于模型组,特别是黄芩甙+灯盏花素1组和黄芩甙组。免疫组织化学方法:增殖细胞核抗原(PCNA)中药干预组(黄芩甙+灯盏花素1组、黄芩甙+灯盏花素2组、BC组、EB组、SM组、GP组)与正常对照组之间增殖细胞阳性率的差别无统计学意义(P>0.05);癌变模型组与中药干预组(黄芩甙+灯盏花素1组、黄芩甙+灯盏花素2组、BC组、EB组、SM组、GP组)、正常对照组之间增殖细胞阳性率的差别均有统计学意义(P<0.01),中药干预组间两两比较,增殖细胞阳性率的差别无统计学意义(P>0.05);检测细胞凋亡率显示:经方差分析,各组间有统计学差别(F=5.07,P<0.0001)。进一步用SNK法进行两两比较,癌变模型组和正常对照组以及中药干预组之间比较均有统计学差异。结论:黄芩甙+灯盏花素1组能明显降低黏膜上皮异常增生发生率,能有效抑制细胞的过度增殖,有效诱导异常细胞的凋亡,对上皮轻度异常增生有明显的癌化学预防作用。
OBJECTIVE: To investigate 4NQO-induced tongue cancer model in rats treated with gynostemma pentaphyllum (GP), astragalus (BC), salvia miltiorrhiza acid (SM), breviscapine (EB) and compound (astragalus + breviscapine). Conduct a dynamic study to evaluate its efficacy. METHODS: Based on the 4NQO-induced rat tongue cancer model, 150 rats were randomly divided into 8 groups: Gynostemma pentaphyllum group, Astragalus membranaceus group, Salvia miltiorrhiza acid group, Breviscapine group, Radix Astragali+ Breviscapine group 1 group. , Astragalus + Breviscapine 2 group, normal control group, cancerous model group), animals were sacrificed at 12~16 weeks, histopathological method and immunohistochemical method (proliferating cell nuclear antigen) were used to detect tissue specimens, and SAS statistics were used. The software performs data processing and analysis of variance for statistics. Results: Histopathological results showed that the degree of epithelial dysplasia in the Chinese medicine intervention group was significantly lower than that in the model group, especially in the group of Huangqi + Breviscapine 1 and Huangqi group. Immunohistochemistry: Proliferating Cell Nuclear Antigen (PCNA) Traditional Chinese Medicine Intervention Group (Astragalus + Breviscapine 1 Group, Astragalus + Breviscapine 2 Group, BC Group, EB Group, SM Group, GP Group) and Normal Control Group There was no significant difference in the positive rate of interproliferating cells (P>0.05); cancerous model group and Chinese medicine intervention group (Huangqi + Breviscapine 1 group, Huangqi + Breviscapine 2 group, BC group, EB group, SM The difference in the positive rate of proliferating cells between the group and the GP group and the normal control group was statistically significant (P<0.01). There was no significant difference in the positive rate of proliferating cells between the two groups of Chinese medicine intervention group (P>0.05). ); Detection of apoptosis rate showed: by analysis of variance, there was a statistical difference between the groups (F = 5.07, P <0.0001). The SNK method was used to compare the differences between the cancer model group and the normal control group and the traditional Chinese medicine intervention group. Conclusion: Astragalus and breviscapine group 1 can significantly reduce the incidence of dysplasia of mucosal epithelium, effectively inhibit the excessive proliferation of cells, effectively induce the apoptosis of abnormal cells, and have a significant cancer chemopreventive effect on mild hyperplasia of the epithelium.