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研究斑蝥水提液中斑蝥素与斑蝥酸在不同条件下的稳定性及转化规律。采用UPLC-TQ-MS分别测定不同pH、不同温度及光照条件下斑蝥水提液中斑蝥素与斑蝥酸的含量。结果显示,斑蝥水提液中斑蝥素的含量随pH的上升而逐渐降低,而其中斑蝥酸的含量变化则相反;斑蝥水提液于不同pH条件下,分别置于25,40℃及25℃光照90 d,定时取样分析,结果显示其中斑蝥素与斑蝥酸在起始的10 d内含量变化较大,具体表现为酸性溶液中斑蝥酸含量的下降与斑蝥素含量的上升,碱性溶液中斑蝥酸含量的上升与斑蝥素含量下降。此后,各条件下两者的含量基本不变。研究表明pH是影响斑蝥水提液中斑蝥素与斑蝥酸含量的主要因素,两者随溶液p H的改变而相互转化,高温与光照可加快这种转化的平衡速度。该研究结果可为以斑蝥药材为原料的制剂的制备工艺质量控制提供一定的参考。
To study the stability and transformation of cantharidin and cantharidin under different conditions in water extract of cantharidin. UPLC-TQ-MS was used to determine the content of cantharidin and cantharidin in water extract of cantharidin at different pH, temperature and light conditions. The results showed that the content of cantharidin in aqueous extract of canthaalta was decreased with the increase of pH, but the content of cantharidin was opposite. The aqueous extracts of cantharidin were placed at 25, 40 and 25 ℃ respectively under different pH conditions The results showed that the content of cantharidin and cantharidin varied greatly in the first 10 days, which showed that the content of cantharidin in acidic solution decreased and the content of cantharidin increased in alkaline solution The increase of cantharidic acid content and the decrease of cantharidin content. Since then, the content of the two under the conditions of the same. Studies have shown that pH is the main factor that affects the cantharidin and cantharidin content in the water extract of cantharidin. Both of them are transformed with the change of solution p H, and the high temperature and light can speed up the balance of this conversion. The results of this study may provide some reference for the preparation process quality control of preparations of Chinese medicinal materials.