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采用ISSR标记技术,对8种桦树(Betula)进行了亲缘关系的分析,17个ISSR引物共检测到236个位点,多态条带比率(PPB)在5.93%～19.92%之间,遗传分化最高的是油桦,最低的是棘皮桦。8种桦树的基因多样性(Ht)为24.38%,桦树种间变异占总变异(Gst)的79.36%。遗传距离聚类将8种桦树分为3个群,黑桦、油桦、白桦和欧洲白桦为一群;赛黑桦、枫桦和岳桦为另一群;棘皮桦单独为一群。聚类结果与传统的形态分类一致。图2表4参10。
ISSR markers were used to analyze the genetic relationships of eight Betula species. A total of 236 loci were detected by 17 ISSR primers and the polymorphic bands (PPB) ranged from 5.93% to 19.92% The highest differentiation is oil birch, the lowest is bark. The genetic diversity (Ht) of eight birch species was 24.38%, and that of birch species accounted for 79.36% of the total variation (Gst). The genetic distance clustering divides the eight birch trees into three groups: black birch, oil birch, white birch and European birch; the black birch, Betula aurea and Yue birch are the other group; The clustering results are consistent with the traditional morphological classification. Figure 2 Table 4 Reference 10.