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[目的]大蓟有广泛的生物活性,具有生物农药的开发潜力。确定其中抗植物病原菌的主要活性成分,以期为其在农药领域的开发利用提供依据。[方法]通过溶剂提取法提取,用生长速率法测定提取物对5种植物病原真菌的抑菌活性,并用柱层析法分离活性物质。[结果]在质量浓度50 g/L时石油醚提取物对石榴枯萎病菌的抑菌活性最高,抑制率达100%,EC50值为5.1 g/L;从中分离出一个三萜类单体化合物,经鉴定为3β,21β-dihydroxyl-20(30)-en-taraxastane。[结论]大蓟对植物病原真菌有良好的抑菌活性,所分离到的单体化合物为大蓟中的主要抑菌活性成分。
[Objective] The thistle has a wide range of biological activity, with the potential for the development of biological pesticides. Identify the main active ingredients against plant pathogens, in order to provide a basis for its development and utilization in the field of pesticides. [Method] The antibacterial activities of the extracts against five plant pathogenic fungi were determined by the method of solvent extraction and the active substances were separated by column chromatography. [Result] The petroleum ether extract had the highest antibacterial activity against Fusarium oxysporum at the concentration of 50 g / L with the inhibition rate of 100% and EC50 value of 5.1 g / L. A triterpene monomer was isolated from the extract, It was identified as 3β, 21β-dihydroxyl-20 (30) -en-taraxastane. [Conclusion] Artemisia annua has good antibacterial activity against phytopathogenic fungi. The isolated monomer compounds are the main antibacterial active ingredients in Artemisia annua.