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目的:探索低剂量电离辐射对LAK细胞治疗动物骨肉瘤的影响。方法:培养的UMR-106细胞接种在月龄大鼠的前胸壁皮下,建立动物骨肉瘤模型。将LAK细胞分别经不同剂量X线辐照后,分别于3、6、9、12、15、18和21d进行细胞计数。采用3H-TdR释放法测定LAK细胞的杀伤活性。winn氏测定法测定LAK细胞对皮下肿瘤细胞生长的影响。结果:大鼠肿瘤发生率达到100%,动物模型建立成功。LAK细胞在第6～9天时,3.25mGy受照组细胞扩增量明显大于对照组。经0.65、3.25mGy辐照及未辐照的LAK细胞输入荷瘤小鼠后,其生存期以3.25mGy组为最长。将骨肉瘤细胞与LAK细胞一并注入小鼠右后肢皮下,30d后发现,3.25mGy辐射的LAK细胞小鼠未见肿瘤生长,而0及0.65mGy辐照的LAK细胞小鼠,可见质量2.0g肿瘤生长,对照组为6.25g。结论:低剂量电离辐射可以使LAK细胞扩增,低剂量电离辐射后LAK细胞的杀伤活性及对骨肉瘤的抗瘤作用均明显增强。
Objective: To explore the effect of low dose ionizing radiation on osteosarcoma in animal treated with LAK cells. Methods: The cultured UMR-106 cells were inoculated subcutaneouly into the anterior wall of the thoracic rats to establish the animal osteosarcoma model. LAK cells were irradiated by different doses of X-ray, respectively, at 3,6,9,12,15,18 and 21d for cell counting. The killing activity of LAK cells was determined by 3H-TdR release assay. The Effect of LAK Cells on the Growth of Subcutaneous Tumor Cells by Winn ’s Assay. Results: The incidence of tumor in rats reached 100%. The animal model was established successfully. LAK cells in the first 6 to 9 days, 3.25mGy exposure group was significantly larger than the control group. The survival time of LAK cells irradiated with 0.65,3.25mGy and non-irradiated LAK cells was the longest in 3.25mGy group. Osteosarcoma cells and LAK cells were injected subcutaneously into the right hind limbs of mice. After 30 days, LAK cells irradiated with 3.25mGy showed no tumor growth, while LAK cells irradiated with 0 and 0.65mGy showed mass of 2.0g Tumor growth, the control group was 6.25g. CONCLUSION: LAK cells can be expanded by low dose ionizing radiation. The killing activity of LAK cells and anti-tumor effect of osteosarcoma after low-dose ionizing radiation are obviously enhanced.