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为探明喀斯特地区丛枝菌根真菌(AMF)的遗传多样性特征,利用巢式PCR和DGGE相结合的分子生物学方法对茂兰喀斯特多个植被类型下的AMF遗传多样性进行了研究。结果表明,喀斯特地区AMF遗传多样性指数和物种丰富度分别平均为3.50和41,远高于非喀斯特对照样地的2.68和17,分析表明,喀斯特地区较高的AMF多样性与此地区丰富的植物多样性以及特殊的生态环境有关,是与喀斯特生态系统长期相互选择的结果。不同植被类型下的AMF多样性差异显著,相似性指数最高为0.34,喀斯特地区AMF的群落结构随着植被类型的改变发生显著变化;基因测序显示,喀斯特地区AMF的优势菌属是生态适应性很强的球囊霉属,在喀斯特石漠化生态恢复中具有较强的利用潜力。
In order to investigate the genetic diversity of arbuscular mycorrhizal fungi (AMF) in karst area, the genetic diversity of AMF under multiple vegetation types in Maolan Karst was studied by nested PCR and DGGE molecular biology methods. The results showed that the average genetic diversity and species richness of AMF in karst areas were 3.50 and 41, respectively, which were much higher than those in non-karst control areas (2.68 and 17). The analysis showed that the higher AMF diversity in karst areas was Plant diversity and special ecological environment are the result of long-term mutual selection with karst ecosystems. The diversity of AMF under different vegetation types was significant, and the highest similarity index was 0.34. The community structure of AMF in karst area changed significantly with the change of vegetation types. The genetic sequencing showed that the dominant species of AMF in karst area were highly ecological adaptability Strong Glomus genus, in karst rocky desertification ecological restoration has a strong potential for utilization.