近年来,由于传染病持续威胁人类健康以及科技的发展,已有可能用新方法解决老问题.因而,重新激起人们对发展疫苗的兴趣与积极性,其中大部分工作是发展抗细菌毒素所致疾病的菌苗(应用经典的方法或新近发展的基因工程技术)和/或毒素亚单位(由基因工程、多肽合成或化学修饰所衍生).本文综述这些新方法,并说明它们在预防几种毒素所致细菌性疾病中的应用, In recent years, new ways of solving old problems have been possible because of the continuing threat to human health and the development of science and technology. As a result, there is renewed interest and enthusiasm in developing vaccines, most of which are due to the development of anti-bacterial toxins (Using classical or newly developed genetic engineering techniques) and / or toxin subunits (derived from genetic engineering, polypeptide synthesis, or chemical modification) of the disease.This paper reviews these new methods and shows their potential role in the prevention of several Toxin-induced bacterial diseases,