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以“紫抗1号”“紫抗2号”“四海升平”3个不同抗性的紫薇品种为试材,采用孢子悬浊液侵染法对试材进行病菌接种,测定了其接种后不同时间(24、48、72、96、120h)内叶片的超氧化物歧化酶(SOD)活性,可溶性蛋白质、丙二醛(MDA)含量,以明确不同侵染时间下紫薇叶片中超氧化物歧化酶活性,可溶性蛋白质、丙二醛含量的变化规律,为白粉病抗性机制的研究提供依据。结果表明:抗病与免疫品种的SOD活性高于感病品种,且抗病与免疫品种的SOD修复快于感病品种;感病品种MDA含量不断上升,抗病品种与免疫品种的含量与变化趋势相近,变化较小,并始终低于感病品种;不用品种的可溶性蛋白质含量变化趋势基本一致,呈先上升后下降的趋势。
The test materials were inoculated with three different crape myrtle cultivars, “Ziqi No.1”, “Ziqi2” and “Sihai Shengping”, using spore suspension inoculation method. The activities of superoxide dismutase (SOD), soluble protein and malondialdehyde (MDA) in leaves at different times (24, 48, 72, 96 and 120h) The changes of superoxide dismutase activity, soluble protein and malondialdehyde content in leaves were analyzed to provide basis for the study on the resistance mechanism of powdery mildew. The results showed that the SOD activity of resistant and immune cultivars was higher than that of susceptible cultivars, and SOD of resistant and immune cultivars was faster than that of susceptible cultivars. MDA content of susceptible cultivars increased continuously, and the contents and changes of resistant cultivars and immune cultivars The trend is similar, the change is small, and always lower than the susceptible varieties; un-cultivated varieties of soluble protein content trends are basically the same, was the first to rise and then decline.