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目的:对瘤果黑种草全草中芦丁的含量进行测定。方法:采用HPLC法,甲醇-0.4%磷酸水溶液(40∶60)为流动相,检测波长:360nm,外标法定量。结果:芦丁在0.02~0.1μg范围呈良好线性关系(r=0.9997)平均回收率=95.1%,RSD=1.4%(n=5)。结论:HPLC方法测定瘤果黑种草中芦丁含量,结果准确可行。
OBJECTIVE: To determine the content of rutin in the whole plant of Malus robustus. Methods: The mobile phase was methanol - 0.4% phosphoric acid solution (40:60). The detection wavelength was 360 nm and the external standard method was used for quantification. RESULTS: Routine showed a good linearity in the range of 0.02-0.1 μg (r = 0.9997). The average recovery was 95.1% with RSD of 1.4% (n = 5). Conclusion: HPLC method for the determination of ruthenium content in black seed germplasm with accurate and feasible results.