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用RP－HPLC法测定射手和鸢尾中射干甙、鸢尾甙的含量．色谱柱以十八烷基键合硅胶为填料，流动相为甲醇－0．2％磷酸溶液（23：77），检测波长265nm。射干甙、鸢尾甙的平均回收率分别为99．40％、100．23％，RSD分别为2．6％、2．2％。结果射干中含射干甙0．24％、鸢尾甙0．35％，鸢尾中含射干甙2．66％、鸢尾甙0．07％。该方法操作简单，结果准确可靠。
RP-HPLC was used to determine the contents of shoot and dovetail in shooter and appendix. The chromatographic column was filled with octadecyl bonding silica gel, and the mobile phase was methanol-0.2% phosphoric acid solution (23:77). The detection wavelength was 265 nm. The average recoveries of sputum and iris were 99.40% and 100.23%, respectively, with RSDs of 2.6% and 2.2%, respectively. The results showed that dry shoots contained 0.24% of sputum, 0.35% of dovetail, and 2.66% of sputum and 0.07% of dovetail. The method is simple and the result is accurate and reliable.