论文部分内容阅读
目的探讨蛋白激酶C epsilon(protein kinase C epsilon,PKC-ε)及碱性调宁蛋白(h1 calponin,h1 CaP)在妊娠小鼠子宫平滑肌细胞中总蛋白和磷酸化蛋白的时空表达变化及与足月临产、早产的关系和机制。方法建立小鼠酒精灌胃早产模型。按妊娠阶段的不同,将小鼠分为未孕组、早孕组(孕7 d)、中孕组(孕12 d)、晚孕组(孕18 d)、足月产组和早产组,每组各10例。应用Western blot检测子宫平滑肌中PKC-ε、磷酸化PKC-ε(pPKC-ε)、h1 CaP、磷酸化h1 CaP(ph1CaP)的蛋白表达。结果足月产组和早产组PKC-ε蛋白表达均显著高于未孕、早孕、中孕组(P<0.01),晚孕组、足月产组、早产组相比较差异无统计学意义(P>0.05);足月产组和早产组h1 CaP蛋白表达均显著高于未孕、早孕、中孕组(P<0.01),晚孕组、足月产组、早产组相比较,差异无统计学意义(P>0.05);足月产组和早产组pPKC-ε/PKC-ε值均显著高于未孕、早孕、中孕组及晚孕组(P<0.01),足月产组与早产组相比较差异无统计学意义(P>0.05);足月产组和早产组ph1CaP/h1 CaP值均显著高于未孕、早孕、中孕组及晚孕组(P<0.01),足月产组与早产组相比较差异无统计学意义(P>0.05);pPKC-ε/PKC-ε的变化与ph1 CaP/h1 CaP的变化呈正相关(r=0.73,P<0.05)。结论 PKC-ε及h1 CaP蛋白表达在临产前即达到最高峰,pPKC-ε及ph1 CaP水平在临产时达最高峰。PKC-ε可通过激活h1 CaP参与足月产及早产的分娩发动。
Objective To investigate the spatial and temporal expression of total protein and phosphorylated protein in the pregnant mouse uterine smooth muscle cells and to explore the relationship between the expression of phosphorylated protein and protein kinase C epsilon (PKC-ε) and h1 calponin (h1 CaP) Monthly labor, the relationship between preterm birth and mechanism. Methods The mouse model of intragastric administration of ethanol was established. According to different stages of pregnancy, the mice were divided into non-pregnant group, pregnant group (7d), pregnant group (12d pregnant), pregnant group (18d), full-term group and preterm group Group of 10 cases. The protein expression of PKC-ε, phosphorylated PKC-ε (pPKC-ε), h1 CaP, phosphorylated h1 CaP (ph1CaP) in uterine smooth muscle was detected by Western blot. Results The expressions of PKC-ε protein in full-term and pre-term groups were significantly higher than those in non-pregnant, early pregnant and middle-aged pregnant women (P <0.01). There was no significant difference between late pregnancy group, full- P> 0.05). The expression of h1 CaP protein in full-term and pre-term groups was significantly higher than that in non-pregnant, early pregnant and pregnant groups (P <0.01) (P> 0.05). The values of pPKC-ε / PKC-ε in full-term and pre-term groups were significantly higher than those in non-pregnant, early pregnant, pregnant women and pregnant women (P <0.01) Compared with preterm group, there was no significant difference (P> 0.05); ph1CaP / h1 CaP of full-term group and premature group were significantly higher than those of non-pregnant group, early pregnancy group, There was no significant difference between full-term group and preterm group (P> 0.05). The change of pPKC-ε / PKC-εwas positively correlated with the change of ph1 CaP / h1 CaP (r = 0.73, P <0.05). Conclusion The expression of PKC-ε and h1 CaP protein peaked before labor, and the peak of pPKC-ε and ph1 CaP levels reached the peak during labor. PKC-ε can be involved in full-term and premature delivery through the activation of h1 CaP.