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目的研究联合应用中药健脾理气方和重组白细胞介素－２（ｒＩＬ－２）对肿瘤化学治疗免疫抑制的影响。方法用环磷酰胺（ＣＴＸ）给Ｈ２２荷瘤小鼠化疗，造成免疫抑制，应用中药健脾理气方和ＩＬ－２联合治疗后，用３Ｈ－ＴｄＲ释放法检测荷瘤小鼠的ＮＫ、ＬＡＫ细胞活性，３Ｈ－ＴｄＲ掺入法检测脾细胞增殖活性，ＦＡＣＳ法检测ＩＬ－２受体，Ｌ３Ｔ４，ＬＹ２阳性细胞数。结果采用大剂量ＣＴＸ后，荷瘤鼠脾重、脾细胞数、脾细胞增殖及杀伤能力均明显降低。Ｔ细胞亚群中ＴＨ比例下降明显，ＩＬ－２受体表达能力也受损，而加用健脾理气方或ｒＩＬ－２后，上述各项指标均有一定改善，其中中药组ＴＨ比例升高较明显；两者合用，则各项免疫指标的恢复更加显著。结论中药及生物反应调节剂联合应用有可能会更有效地减轻化疗的免疫抑制作用。
Objective To study the effect of combined use of Chinese medicine Jianpi Liqi Recipe and recombinant interleukin-2 (rIL-2) on immunosuppression of tumor chemotherapy. METHODS: Cyclophosphamide (CTX) was given to H22 tumor-bearing mice to induce immunosuppression. NK and LAK cells in tumor-bearing mice were detected by 3H-TdR release after combined treatment of Jianpi Liqi Recipe and IL-2. Activity, 3H-TdR incorporation assay was used to detect the proliferation of spleen cells. FACS was used to detect the number of IL-2 receptor, L3T4, LY2 positive cells. Results After high-dose CTX was used, the spleen weight, the number of splenocytes, and the proliferation and killing capacity of spleen cells in the tumor-bearing mice were significantly reduced. The proportion of TH in the T cell subpopulation decreased significantly, and the IL-2 receptor expression ability was also impaired. After the addition of Jianpi Liqi Recipe or rIL-2, all of the above indicators were improved. The proportion of TH in the Chinese medicine group increased. Obviously; the combination of the two, the recovery of the various immune indicators is more significant. Conclusion The combined use of traditional Chinese medicine and biological response modifiers may reduce the immunosuppressive effect of chemotherapy more effectively.