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为了探讨严重急性呼吸综合征(SARS)冠状病毒(SARS-CoV)抗体测定在系统性红斑狼疮(SLE)患者中的假阳性问题,应用酶联免疫吸附试验(ELISA)和荧光定量RT-PCR技术检测了66例正常对照和31例SLE患者血清中SARS-CoV抗体的阳性率。结果,66例正常对照中,IgM抗体均阴性,IgG抗体的阳性率为3.0%(2/66);31例SLE患者中,IgM抗体和IgG抗体阳性率分别为29%(9/31)和58.1%(18/31),IgG抗体和IgM抗体同时阳性为22.6%(7/31)。经RTPCR检测,上述阳性病例均为阴性。结论:用非纯化抗原制备的ELISA试剂盒测定SLE患者的SARS-CoV抗体,可能出现假阳性,两种抗体同时测定可降低诊断的假阳性率,提高诊断的特异性。在SLE患者中出现假阳性的原因可能与包被的抗原有关。
In order to investigate the false positives of SARS-CoV antibodies in patients with systemic lupus erythematosus (SLE), enzyme linked immunosorbent assay (ELISA) and fluorescent quantitative RT-PCR The positive rates of SARS-CoV antibody in sera of 66 normal controls and 31 SLE patients were detected. The positive rate of IgG antibody was 3.0% (2/66) in 66 normal controls. The positive rates of IgM antibody and IgG antibody in 31 SLE patients were 29% (9/31) and 58.1% (18/31), IgG antibody and IgM antibody positive at 22.6% (7/31). The RTPCR test, the positive cases were negative. CONCLUSIONS: ELISA kits prepared with non-purified antigens may be used to detect SARS-CoV antibodies in patients with SLE. False positives may be found in these SLE patients. Simultaneous determination of the two antibodies may reduce the false-positive rate of diagnosis and improve the diagnostic specificity. The reason for the false positives in SLE patients may be related to the coated antigen.