胰腺癌发病隐匿 ,是预后最差的恶性肿瘤之一。如能在发病初期及时、准确地作出判断 ,将大大增加手术切除的机会 ,提高 5年生存率 ,改善预后。ｒａｓ基因家族包含Ｈ ｒａｓ、Ｋ ｒａｓ和Ｎ ｒａｓ 3种功能基因。近年来研究发现 ,胰腺癌Ｋ ｒａｓ基因第 12密码子点突变率高达 6 0 Incidence of pancreatic cancer is one of the worst malignant tumors. If we can make a timely and accurate judgment at the early stage of disease, we will greatly increase the chance of surgical resection, improve the 5-year survival rate, and improve the prognosis. The ras gene family contains three functional genes: H ras, Kras and N ras. In recent years, it has been found that the mutation rate of the 12 th codon point of pancreatic cancer Kras gene is as high as 60