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目的应用分子分型技术对2007年上海和重庆市沙门菌监测点的50株汤卜逊沙门菌进行分子流行病学分析和抗生素敏感性测定,了解上海和重庆两地菌株的分子分型特征和药物敏感性特征。方法抗生素敏感性测定采用微量肉汤稀释法,分子分型方法包括脉冲场凝胶电泳(PFGE)和多位点串联重复序列分析(MLVA)。结果药敏试验显示78%的菌株存在多重耐药,其中磺胺甲恶唑和四环素的耐药率最高,其次是甲氧苄胺嘧啶;对头孢类抗生素(头孢噻肟、头孢三嗪、头孢他啶、头孢噻呋)均未产生耐药性。PFGE将50株菌分为15个带型,30株重庆分离株间有较高的相似性;MLVA分析显示除Sal16位点外,其余检测位点在所有待检菌株中没有差别。结论分子分型支持汤卜逊沙门菌引起的暴发以及散发。目前MLVA分型应用于汤卜逊沙门菌分子分型时,分型能力低于PFGE,需要进一步优化。
Objective To analyze molecular epidemiology and antibiotic susceptibility of 50 strains of Salmonella typhimurium in Shanghai and Chongqing in 2007 by molecular typing technique and to understand the molecular typing characteristics of strains in Shanghai and Chongqing Drug sensitivity characteristics. Methods The antibiotic sensitivity was determined by micro broth dilution method. The molecular typing methods included pulsed-field gel electrophoresis (PFGE) and multi-site tandem repeat analysis (MLVA). Results The susceptibility test showed that 78% of the isolates were multi-drug resistant. Among them, sulfamethoxazole and tetracycline had the highest drug resistance rates, followed by trimethoprim, cefotaxime, cefotaxime, ceftazidime, Ceftiofur) did not produce drug resistance. 50 isolates of PFGE were divided into 15 bands and 30 strains of Chongqing isolates had higher similarity. MLVA analysis showed no difference among all tested isolates except Sal16. Conclusion Molecular typing supports the outbreak and dissemination of Salmonella typos. At present, when MLVA typing is applied to the molecular typing of Salmonella Thompson, the typing ability is lower than that of PFGE and further optimization is needed.