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目的:建立同时测定灯盏细辛提取物中野黄芩苷,3,5-二-O-咖啡酰奎宁酸和3,4-二-O-咖啡酰奎宁酸的含量测定方法。方法:采用高效液相色谱法,大连依利特C18色谱柱(4.6mm x 250 mm,5μm),流动相乙腈-0.1%磷酸(22∶78),检测波长330 nm,流速1 m L·min-1,柱温35℃。结果:野黄芩苷,3,5-二-O-咖啡酰奎宁酸和3,4-二-O-咖啡酰奎宁酸分别在0.032 7~1.308 0μg(r=0.999 9),0.048 2~1.928 0μg(r=0.999 9),0.058 3~2.332 0μg(r=0.999 8)呈良好的线性关系,加样回收率分别为99.74%(RSD=1.9%),100.23%(RSD=1.4%),99.33%(RSD=1.3%)。结论:本法简便、快捷,结果准确、重复性好,可用于灯盏细辛提取物中3种有效成分的含量测定。
Objective: To establish a method for the simultaneous determination of wild baicalin, 3,5-di-O-caffeoylquinic acid and 3,4-di-O-caffeoylquinic acid in Erigeron Breviscapus. Methods: The HPLC assay was performed on a Dalian Ellite C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile-0.1% phosphoric acid (22:78) at a detection wavelength of 330 nm and a flow rate of 1 mL · min- 1, column temperature 35 ℃. Results: The concentrations of wild baicalin, 3,5-di-O-caffeoylquinic acid and 3,4-di-O-caffeoylquinic acid were in the range of 0.032 7-1.308 0μg (r = 0.999 9) 1.928 0μg (r = 0.999 9), 0.058 3 ~ 2.332 0μg (r = 0.999 8). The recoveries were 99.74% (RSD = 1.9%) and 100.23% (RSD = 1.4% 99.33% (RSD = 1.3%). Conclusion: The method is simple, rapid, accurate and reproducible. It can be used to determine the content of three effective components in the volatile oil from Erigeron Breviscapus.