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目的：利用全自动生化分析仪，建立一种快速检测血清总胆汁酸（ＴＢＡ）活性的方法和正常参考值。方法：双试剂酶偶联连续监测法，检测６０例不同肝病患者和３００名健康成人血清ＴＢＡ活性。结果：其测值（ｘ±ｓ）分别为４８．９５±５．７３μｍｏｌ／Ｌ和５．１２±３．４６μｍｏｌ／Ｌ，差异有显著性（Ｐ＜０．０１）；本法批内ＣＶ分别为１．９９％、２．８５％和３．００％，批间ＣＶ分别为３．２８％、３．４０％和３．１６％，线性范围０～２００μｍｏｌ／Ｌ。结论：双试剂酶偶联连续监测法，线性好，特异性强，灵敏度高，快速、准确，对肝脏疾病的诊断及临床分级具有重要价值。
OBJECTIVE: To establish a rapid detection method of serum total bile acid (TBA) activity and normal reference value by using automatic biochemical analyzer. Methods: Serum TBA of 60 patients with different liver diseases and 300 healthy adults were detected by dual-reagent enzyme-linked continuous monitoring method. Results: The measured values (x ± s) were 48.95 ± 5.73μmol / L and 5.12 ± 3.46μmol / L, respectively, with significant difference (P <0.01) Were 1.99%, 2.85% and 3.00% respectively. The inter-assay CV was 3.28%, 3.40% and 3.16%, respectively. The linear range was 0 ~ 200μmol / L. Conclusion: The dual-reagent enzyme-linked continuous monitoring method is of great linearity, specificity, sensitivity, fastness and accuracy and is of great value in the diagnosis and clinical grade of liver diseases.