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本文叙述了应用多波长萤光分光光度法测定人工样品中的萤光黄、罗丹明6G和罗丹明B_。于波长502nm直接测量萤光黄的萤光强度,罗丹明6G和罗丹明B均不干扰;罗丹明6G的测定波长λ_1为555nm,参比波长λ_2和λ_3为502nm和660nm,罗丹明B的测定波长λ_1选用585nm,参比波长λ_2、λ_4和λ_3相应为538nm、508nm和494nm,方法的相对误差一般小于6%。作者于前文叙述了多波长萤光分光光度法的原理,为了验证该原理的可靠性,特对萤光黄、罗丹明6G和罗丹明B人工混合样品进行实测。结果表明,方法原理可靠,实验操作和数据处理简便快速,三次测定平均偏差<3%。
This article describes the determination of luciferase, rhodamine 6G and rhodamine B_ in human samples by multiwavelength fluorescence spectrophotometry. The fluorescence intensity of Lucifer yellow was measured directly at the wavelength of 502 nm, Rhodamine 6G and Rhodamine B did not interfere with each other. The determination wavelength of rhodamine 6G was 555 nm, the reference wavelengths λ 2 and λ 3 were 502 nm and 660 nm, The wavelength λ_1 is selected to be 585 nm. The reference wavelengths λ_2, λ_4 and λ_3 are 538 nm, 508 nm and 494 nm, respectively. The relative error of the method is generally less than 6%. The author described in the previous multi-wavelength fluorescence spectrophotometry principle, in order to verify the reliability of the principle, especially the fluorescent yellow, rhodamine 6G and rhodamine B artificial mixed samples were measured. The results show that the method is reliable, the experimental operation and data processing are simple and quick, the average deviation of three determinations is less than 3%.