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目的:研究西红花酸对巨噬细胞源性泡沫细胞形成的影响。方法:分离培养小鼠腹腔巨噬细胞,与氧化性低密度脂蛋白温孵培养建立泡沫细胞模型。采用高效液相法测定细胞内胆固醇的含量,观察西红花酸对巨噬细胞源性泡沫细胞形成的影响。结果:小鼠腹腔巨噬细胞与20 mg.L-1氧化型低密度脂蛋白作用72 h后,细胞内胆固醇酯占总胆固醇的65.97%,形成泡沫细胞。加入不同浓度的西红花酸后,西红花酸能剂量依赖性地抑制氧化型低密度脂蛋白所致的巨噬细胞胆固醇酯的堆积(P<0.01)。结论:西红花酸能抑制巨噬细胞源性泡沫细胞的形成,这提示西红花酸能减少巨噬细胞内胆固醇酯的堆积可能是西红花酸抗动脉粥样硬化的作用机制之一。
Objective: To study the effect of crocetin on the formation of macrophage-derived foam cells. Methods: Mouse peritoneal macrophages were isolated and cultured, and incubated with oxidized low density lipoprotein to establish a foam cell model. The contents of intracellular cholesterol were determined by HPLC. The effects of crocetin on the formation of macrophage-derived foam cells were observed. Results: After intraperitoneal macrophages were treated with 20 mg.L-1 oxidized low density lipoprotein for 72 h, the intracellular cholesterol ester accounted for 65.97% of the total cholesterol, forming foam cells. After adding different concentrations of crocetin, crocetin dose-dependently inhibited oxidized low density lipoprotein-induced macrophage cholesterol ester accumulation (P <0.01). Conclusion: Crocetin can inhibit the formation of macrophage-derived foam cells, suggesting that crocetin can reduce the accumulation of cholesterol esters in macrophages may be one of the mechanisms of anti-atherosclerosis action of crocetin .