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硝酸还原酶(NADH:NR E.C.1.6.6.1,以下简称NR)是植物体内硝酸盐同化过程中的关键酶。在植物氮素代谢中起着重要作用,它对植物的生长发育有重要的影响。NR的酶量及活力控制着硝态氮转化的速率,不同生长速率类型的树木,NR活力表现不同,速生类型的NR活力要大于生长慢的类型。NR可望成为早期鉴定的指标。林木一般多栽培于山地,立地条件难以做到均一,必然使得各个个体植株的NR活力产生差异。为了正确评价NR活力在不同生长速率类型间的差异(即:在大田条件下,不同生长速率类型无性系植株所表现出的NR活力差异,是本身的特性还是由外界环境,特别是土壤肥力条件所造成。),除了在测定技术上增加诱导处理措施可以克服环境差异外,本文主要研究氮素水平对不同生长速率类型的杉木NR活力的影响,为大田鉴定指标化提供依据。
Nitrate reductase (NADH: NR E.C. 1.6.6.1, hereinafter referred to as NR) is a key enzyme in nitrate assimilation in plants. Plays an important role in the plant nitrogen metabolism, it has an important impact on plant growth and development. NR enzyme activity and vitality control the rate of nitrate conversion, the growth rate of different types of trees, NR vitality performance, fast-acting type of NR activity is greater than the slow-growing type. NR is expected to be an indicator of early identification. Trees generally cultivated in the mountains, site conditions difficult to achieve uniformity, inevitably make each individual plant NR activity differences. In order to correctly evaluate the difference in NR activity among different growth rate types (ie, differences in NR activity exhibited by clonal plants of different growth rates under field conditions are their own characteristics or are caused by the external environment, especially soil fertility conditions ). In addition to the measurement techniques to increase the induction treatment can overcome environmental differences, this paper mainly studies the nitrogen level on the growth of different types of fir NR activity to provide the basis for identification of field identification.