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建立了免疫亲和柱同时净化-高效液相色谱法检测植物油中的黄曲霉毒素B1、B2、G1、G2和玉米赤霉烯酮的分析方法。黄曲霉毒素B1、B2、G1、G2的方法的定量限均为0.1μg/kg,玉米赤霉烯酮的方法的定量限为5.0μg/kg,相对标准偏差低于10.4%,回收率为82.0%~95.5%。该方法简便快速、灵敏准确、重现性好,可以用于对植物油中黄曲霉毒素B1、B2、G1、G2和玉米赤霉烯酮进行快速定量检测。
A method for simultaneous determination of aflatoxins B1, B2, G1, G2 and zearalenone in vegetable oils by immunoaffinity column simultaneous purification and high performance liquid chromatography was established. The limit of quantification for aflatoxin B1, B2, G1, G2 was 0.1 μg / kg, the limit of quantification for zearalenone was 5.0 μg / kg, the relative standard deviation was less than 10.4% and the recovery was 82.0 % ~ 95.5%. The method is simple and rapid, sensitive and accurate, reproducible and can be used for rapid and quantitative determination of aflatoxins B1, B2, G1, G2 and zearalenone in vegetable oils.