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应用DNA重组、基因转染、分子杂交、生长测定、裸鼠成瘤、凝胶迁移率改变分析等方法观察了人胃癌细胞中原癌基因erbB-2的表达被其反义RNA特异抑制后胃癌细胞恶性增殖、EGF反应性及相关基因表达的变化.erbB-2表达下调显著抑制了胃癌细胞的恶性增殖能力、EGF的细胞增殖促进效应及对增殖相关基因c-myc,EGFR的促进效应.与此同时,细胞周期蛋白激酶抑制物p21基因的表达显著增强.对其转录激活机制分析结果表明,转录激活分子STAT1特异性地与p21基因启动子结合促进了p21基因的表达.
The expression of the proto-oncogene erbB-2 in human gastric cancer cells was specifically inhibited by its antisense RNA after the application of DNA recombination, gene transfection, molecular hybridization, growth assay, tumor formation in nude mice, and analysis of changes in gel mobility. Malignant proliferation, EGF reactivity and related gene expression changes. Down-regulation of erbB-2 expression significantly inhibited the malignant proliferation of gastric cancer cells, EGF cell proliferation promoting effect and the promotion of proliferation-related genes c-myc, EGFR. At the same time, the expression of cyclin kinase inhibitor p21 gene was significantly enhanced. The transcriptional activation mechanism analysis showed that the transcriptional activation molecule STAT1 specifically binds to the p21 gene promoter to promote the expression of p21 gene.