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1982—1986年先后从山东、河南、安徽、云南、贵州、福建、湖南、湖北、陕西、四川和辽宁等省采集并分离到烟草黑胫病菌系255个;根据鉴别寄主的病害反应鉴定其生理小种:用游动孢子悬浮液注射接种10叶期左右鉴别寄主的茎或茎基部。所用的鉴别寄主有白肋烟品系 L_8,烤烟品种 N.C.10 71和小黄金10 25等,及烟草野生种N.nudicaulis。结果是,有231个菌系对 L_8、N.C.10 71和 N.nudicaulis 无致病力或仅有弱致病力,应为0号小种;有12个菌系对四个鉴别寄主都有高度致病力,应为1号小种:有12个菌系对 L_8和 N.C.10 71无致病力,而对 N.nu-dicaulis 有中到高度致病力,根据它们的病害反应不像是0、1和3号小种,是否是2号小种还是新的小种有待进一步研究。参照田间检测结果,我们初步认为我国主要烟区烟草黑胫病菌群体中,0号小种为优势小种,亦有少量1号小种的存在,而在辽宁省1号小种似乎比0号小种更重要一些,在11个菌系中,1号小种7个,0号小种4个。
A total of 255 tobacco blackleg pathogenic bacteria strains were collected and isolated from Shandong, Henan, Anhui, Yunnan, Guizhou, Fujian, Hunan, Hubei, Shaanxi, Sichuan and Liaoning Provinces from 1982 to 1986; their physiological Race: Inoculated with zoospore suspension inoculated about 10 leaves to identify the host stem or stem base. The identification of the host used are burley variety L_8, tobacco varieties N.C.10 71 and small gold 10 25, and tobacco wild species N. nudicaulis. As a result, 231 strains had no virulence or weak virulence to L_8, NC1071 and N. nudicaulis and should be race 0; 12 strains were highly susceptible to all four identified hosts Virulence should be Race 1: 12 strains have no virulence to L_8 and NC10 71, and moderate to high virulence to N. nu-dicaulis, depending on their disease response Races 0, 1 and 3 are minor races or new races for further study. With reference to the field test results, we initially believe that race 0 is the dominant race in tobacco black shank in China, and there is also a small number of race 1. However, it seems that race 1 in Liaoning Province is better than 0 The races are more important. Among the 11 strains, there are 7 race 1 and 4 race 0.