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利用聚丙乙烯酰胺凝胶垂直板电泳法,对红豆草6个品种、扁蓿豆3个品种进行了品种鉴定。根据吸胀种子过氧化物酶同工酶电泳图谱类型和酶活性强弱,可将红豆草的6个品种逐个鉴别出来,但对扁蓿豆3个品种鉴别效果不够明显;根据吸胀种子的酯酶同工酶电泳图谱类型,可将9个品种逐个鉴定出来,以酯酶活性强弱鉴定这9个品种效果最好。
The polyacrylamide gel vertical plate electrophoresis method was used to identify 6 varieties of sainfoin and 3 varieties of alfalfa. According to the type and activity of peroxidase isozymogram of imbibed seeds, six varieties of sainfoin could be identified one by one, but the identification effect of three varieties of alfalfa was not obvious. According to the characteristics of swollen seeds Esterase isoenzyme electrophoresis pattern types, one by one can be identified nine varieties out to esterase activity of the nine varieties identified the best effect.