拮抗桃树根癌病菌株的筛选

来源 :上海农业学报 | 被引量 : 0次 | 上传用户:xrp880823
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桃树根癌病(Crown gall)是由放射形土壤杆菌根癌变种(Agrobacterium radiobacter Var. tumefaciens)引起的植物细菌病害,澳大利亚Kerr运用放射土壤杆菌K84(A.radiobacter K84)成功地防止了该病害的发生和蔓延。我国土壤杆菌的研究偏重于遗传方面。本文报道一种简便、快速的筛选方法。从上海市郊分离的100株菌株,通过番茄结瘤试验和平皿相互拮抗试验获得1-1-4和1-1-9等两株有效菌株;生物统计结果表明,本文试验的结果与Kerr的报道相符。 Crown gall is a plant bacterial disease caused by the Agrobacterium radiobacter var. Tumefaciens, and Kerr, Australia, successfully prevented the disease with A.radiobacter K84 The occurrence and spread. The research of Agrobacterium in our country focuses on genetic aspects. This article reports a simple and rapid screening method. 100 isolates isolated from the outskirts of Shanghai were used to obtain two effective strains, 1-1-4 and 1-1-9, by means of nodulation test and plate antagonistic test. The results of bioassay showed that the results of this study were consistent with those reported by Kerr Match.
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