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经分离、接种证实，烟草种子上带有烟草赤星病菌Ａｌｔｅｒｎａｒｉａａｌｔｅｒｎａｔａ、烟草野火病菌Ｐｓｅｕｄｏｍｏｎａｓｓｙｒｉｎｇａｅｐｖ．ｔａｂａｃｉ；带菌率随品种和发病程度而异。赤星病菌一般为１８％左右，野火病菌为８％～１６％；此外，种子上还有镰刀菌Ｆｕｓａｒｉｕｍｓｐｐ．和一些杂菌。赤星病菌和野火病菌不仅种子表面带有，而且种皮内部和胚乳也带有，胚则无菌。对带有这两种病菌的种子进行处理，以温汤浸种５０℃１０分钟、５０℃１５分钟和５２℃１０分钟对赤星病和野火病菌的灭菌效果最好；链霉素浸种对烟草野火菌也有很好的灭菌作用。５０℃、５２℃温汤浸种及链霉素浸种对烟草种子发芽率均没有明显的影响。
After isolation and inoculation confirmed that tobacco seeds with Alternaria alternata Alternariaalternata, tobacco wildfire bacteria Pseudomonas alringtonepv. tabaci; carrier rate varies by species and degree of disease. Allelic pathogens generally about 18%, wildfire bacteria 8% to 16%; In addition, the seeds Fusariumspp. And some bacteria. Alternaria alternata and wildfire are not only present on the seed surface, but also inside the seed coat and endosperm, while the germ is sterile. The seeds with these two kinds of germs were treated with soaking Wen Wen Tang in 50 ℃ for 10 minutes, 50 ℃ for 15 minutes and 52 ℃ for 10 minutes. The results showed that streptomycin There is also a good sterilization. No significant effect on the germination rate of tobacco seeds was observed at 50 ℃ and 52 ℃ soak seeds and streptomycin soaking seeds.